MACRO, Stereo, Brightfield (RGB color, epifluorescence)

FRANK - Zeiss Discovery.V12 fluorescent stereo

Light Source & Filters

Light Sources:

  • Fostec Fiber Optic halogen illumination

Standard fluroescent filter configurations:

  • GFP, AzamiGreen, Emerald, etc.

  • mRFP, mCherry, TdTomato, etc.

• 1X Zeiss NA0.XX objective

• 8X - 100XÌývia motorized optical zoom

• selectable display to indicate field size diameter, resolution

Nikon Digital Sight CCD camera (DS-Qi; 6.45 um x 6.45 um pixel)

  • Fluorescent imaging of GFP and RFP and their related probes over a magnification range.
  • Halogen illumination via Fostec Fiber Optic.

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  • NIS Elements BR 5.20.02 for fluorescence imaging (monochrome)

FRANK, front view
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FRANK, right view

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PEPPER - Nikon TiU widefield with RGB color brightfield

Light Source & Filters

Light Sources:

  • Halogen illumination for brightfield and DIC

Standard filter configurations:

Semrock BrightLine quad Sedat
  • DAPI, Hoechst, etc.
  • Alexa488, FITC, GFP, etc.
  • TRITC, Alexa555, 568, 594, mRFP, mCherry, TdTomato, etc.
  • Alexa647, Cy5, etc.

Standard objectives:

  • 2x 0.10NA Plan Apo WD 8.5Ìý(mm)Ìý
  • 4x 0.13NA Plan FluorÌýWD 17.2 (mm)
  • 10x 0.45NA Plan Apo DIC N1ÌýWD 4 (mm)
  • 20x 0.50NA Plan FluorÌýWD 2.1 (mm)
  • 40xÌý0.60NA Plan FluorÌýWD 1.8Ìý(mm)
  • 100x 1.30NA Plan Fluor Ph3 OIL WD 0.20 (mm)

Optional objectives:

  • 60x 1.40NA Plan Apo VC λ OIL WD 0.13 (mm)
  • More (please inquire)
  • Ìýfor fluorescence
  • Ìýfor color brightfield (e.g. eosin, hematoxylin, crystal violet. etc.). This camera uses a CMOS chip with 5.9 megapixels.Ìý

Ìýmotorized stage. Sample holders for standard slide, 35 and 60 mm dishes, and multiwell plates. DIC possible at 10x, andÌý60x.ÌýBrightfield color RGB imaging.ÌýMulti-point imaging.ÌýMontaging with stitching.ÌýLive cell imaging with TOKAI-HIT Cell Incubator; 1 X 35 (mm) dish or chamber slide (coverslip bottom).ÌýRapid 4 color imaging via quad Sedat filter set (Semrock BrightLine).

PEPPER - Nikon Inverted Widefield Microscope (Front View)

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MACRO - Olympus MVX10 MacroZoom fluorescent & color

Light Source & Filters
  • Built in high-contrast white LED in base for transmission imaging
  • CoolLED pE-300White: 400, 488, 561 nm LEDs
  • ET-DAPI/FITC/TxRed triple Sedat filter set

Objectives (note, 2 position nose piece):

  • Olympus 0.63X MVXPLAPO NA0.15 WD87mm (max field of view 55mm)
  • Olympus 1X MVXPLAPO NA025 WD65mmÌý(max field of view 34.9mm)
  • Olympus 2X MVXPLAPO NA0.50 WD20mmÌý(max field of view 17.6mm)

Zoom body:

  • 0.63 - 6.3X

System magnifier:

  • 2X

Effective magnification (to eyes;Ìý10X less to camera when using 1X coupler and no system magnifier):

  • Total system, 4Ìý- 250X
  • 0.63X objective, 4Ìý- 80X
  • 1X objective, 6.3Ìý- 125X
  • 2X objective 12.5Ìý- 250X

Color RGB camera:

  • , 1x1.8 inch 6.4MP (3088 x 2076 pixels) color CMOS, 2.4 micronÌýx 2.4 micronÌýpixels

Monochrome camera:

  • 1`920 x 1200 pixels) CMOS, 5.86 x 5.86 micron pixels

System features:

  • Sytem Macro and stereo viewing modes; macro maximizes brightness, stereo splits the light path to enhance depth of field
  • Color camera is mounted with 0.63X adapter to maximize field size
  • Monochrome camera is mounted at 1.0X distance, no glass to maximize brightness
  • 2 position nose piece allows 2 objectives to be mounted simultaneouly
  • Diaphragms to adjust brightness for both white light and fluorescece
  • Triggered LED allow efficient blue, green, red imaging
  • Customizable intensity for each LED to limit photobleaching

Capabilities:

  • Base includes brightfield high-contrast, brightfield oblique, darkfield and blocking optics
  • Time-lapse microscopy
  • Multi-channel fluorescence
  • Fiber optic white light (Fostec) for reflected light imaging

Olympus cellSens Standard versionÌý4.2

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MACRO darkfield optics in base

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