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Our approach to super-resolution microscopyÌýis fundamentally different from most existingÌýtechniquesÌýsuch as stimulated emission depletion microscopy, structured illumination microscopy, or single-molecule localization microscopy. For common fluorescent samples, our method typically achieves ~3-5 fold resolution enhancement over theÌý​

Unlike most existing techniques,Ìýour method does not require special fluorophores or additional sample treatments. All it needs is a focused laser spot for illumination, and a constrained deconvolution algorithm.​

The mechanism of our method is aÌýstory that spansÌýnearly a half century. In the early 1970's, Prof. Roy Frieden and his colleagues in the University of ArizonaÌýdemonstrated that certainÌý.ÌýIn the 90's,Ìýseveral mathematiciansÌýpointed outÌýthatÌýtheÌý. Another 20-plusÌýyears later, our groupÌýcoincidentally discoveredÌýthatÌýÌýcompared to the conventional widefield, uniform illumination.​

While achieving superior super-resolution, the true advantages of our method is its simplicity for biological fluorescence microscopy, and its potential for live-cellÌýimaging.

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