Multi-Photon

GEMINI - Olympus FVMPE-RS twin laser multi-photon (Porter B051)

Lasers & Filters

Lasers

This microscope is equipped with two separate, pre-chirped, tunable, pulsed IR lasers. Please click on the links below for further inquiries.Ìý

Emission Filters

Large format, 32mm diameter filters are used.

  • Olympus: ‘violet’ 410-455nm, 'green' 495-540nm, 'red' 575-645nm) and far red 660-750nmÌýemission
  • Chroma: 'cyan' 460-500nmÌýand yellow 520-560nm

Multi-photon optimized objectives include:

  • 5xÌýNA0.10; MPLN5X-1-7 air, WD 20 (mm)
  • 10xÌýNA0.60; XLUMPLFL10XW-SP water immersion, WD 3.3 (mm);Ìýaka 'The Super 10' (this is a special order lens)
  • 20x NA0.45; IR LCPlan N with correction collar, WDÌý8.93 - 8.18Ìý(mm);ÌýAir, Glass or Silicone
  • 25xÌýNA1.05; FV30-AC25W truResolution water immersion, WD 2.0 (mm) with motorized correction collar (Auto CORR)
  • 60xÌýNA1.10;ÌýLUMFLN60XW LWD water immersion, WD 1.5 (mm)
  • Two non-descanned high-sensitivity GaAsP PMTs
  • Two non-descanned increased-sensitivity multi-alkali PMTs

This microscope is housed in an independent, secured room with significant light mitigation and isÌýinside a custom-made dark box. This microscope is excellent for simultaneous multi-color, deep, in situ imaging of excised or live samples, including tissues and whole animals under anesthesia.

  • Olympus Fluoview acquisition software
  • Two pre-chirped, tunable, pulsed IR lasers
    • Laser auto-alignment
    • Beam-shaping for deep focus
    • Beam scanning modes
  • High sensitivity PMTs and optics designed for long wavelength transmittance and reflectance
  • Hybrid scanning with galvanometer and resonance scanners for high quality and fast image acquisition
  • Multi-photon optimized objectives
    • 25xÌýhigh NAÌýlong-working distanceÌýwith real-time motorized correction collar for deep imaging at high resolution and contrast
    • 10xÌýhigh NA long-working distanceÌýobjective (special order) for deep imaging atÌýhigh resolution imaging
    • 20x is optimal for GRIN lens work via NA-matching to implant and long working distance
    • 5xÌýprovides large field of view imaging for entire organs and structures
    • 60xÌýhigh NA withÌýlong-working distance allows resolution of orgelles, chromosomes, etc. in vivo
  • ​Large, motorized platform stage providing space for sample holding and articulation, with pre-drilled mounts
  • Single position nosepiece for 10x and 25x,Ìýdual position nosepiece for 5x, 20x, and 60x
  • TTL for triggered acquisition

Available upon request

  • Stoelting specimen warming pad (ideal for small animals)
  • Isoflurane anesthesia providing long-term imaging of small animals

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GEMINI - Olympus MultiphotonÌý

GEMINI - Olympus Multiphoton without light-proof cover

GEMINI - Olympus Multiphoton with anesthesia, warm pad and 25X lens

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GEMINI - Olympus Multiphoton lasers (Spectra Physics Insight X3, right and MaiTai eHP DeepSee, left)

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GEMINI - Olympus Multiphoton 25X lens

GEMINI - Olympus Multiphoton 60X and 5X lenses

GEMINI - Olympus Multiphoton 10X lens (aka 'The Super 10')